dc.contributor.author |
Ashfaq, M. |
|
dc.contributor.author |
Aslam, A. |
|
dc.contributor.author |
Mustafa, G. |
|
dc.contributor.author |
Danish, M. |
|
dc.contributor.author |
Nazar, M. Faizan |
|
dc.contributor.author |
Asghar, M. Nadeem |
|
dc.date.accessioned |
2018-07-29T08:56:15Z |
|
dc.date.available |
2018-07-29T08:56:15Z |
|
dc.date.issued |
2015 |
|
dc.identifier.issn |
1815-3852 |
|
dc.identifier.uri |
https://journal.uob.edu.bh:443/handle/123456789/1084 |
|
dc.description.abstract |
A viable cost-effective and isocratic approach employing C-18 column (250 mm · 4.6 mm, 5 lm) based HPLC has been utilized to separate and estimate the drug, tranexamic acid in pharmaceutical formulations. Tranexamic acid contains no p-electrons to act as fluorophore or chromophore hence pre-column derivatization was performed with benzene sulfonyl chloride in aqueous medium at room temperature. The derivatized drug was then estimated using C-18 column by exploiting a 25:75 (v/v) solvent mixture of acetonitrile and 0.1 M ammonium acetate (pH 5.0) as the mobile phase. The flow rate of mobile phase was 1 mL/min and detection was performed at a wavelength of 232 nm using UV detector. Retention time of tranexamic acid was 4.42 min. The method followed linear regression equation in the concentration range of 1– 100 lg/mL with co-efficient of determination equal to 0.9994. The limit of detection and limit of quantitation were 0.3 and 1 lg/mL, respectively. The relative standard deviation and recovery ranges for tranexamic acid were found to be 0.11–2.47% and 97.60–103.25%, respectively. The suggested method is very sensitive and may have the potential to be used for tranexamic acid detection in medicinal formulations. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
University of Bahrain |
en_US |
dc.rights |
Attribution-NonCommercial-ShareAlike 4.0 International |
* |
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-sa/4.0/ |
* |
dc.subject |
Tranexamic acid |
|
dc.subject |
Derivatization |
|
dc.subject |
Chromophore |
|
dc.subject |
HPLC |
|
dc.subject |
Validation |
|
dc.title |
Derivatization/chromophore introduction of tranexamic acid and its HPLC determination in pharmaceutical formulations |
en_US |
dc.type |
Article |
en_US |
dc.identifier.doi |
http://dx.doi.org/10.1016/j.jaubas.2014.02.005 |
|
dc.source.title |
Arab Journal of Basic and Applied Sciences |
|
dc.abbreviatedsourcetitle |
AJBAS |
|