University of Bahrain
Scientific Journals

Spectrophotometric determination of pyrimethamine (PYM) in pharmaceutical formulation using 1,2-naphthoquinone-4-sulfonate (NQS)

Show simple item record Elbashir, Abdalla A. Elwagee, Alawia H.E. 2018-07-26T06:35:59Z 2018-07-26T06:35:59Z 2012
dc.identifier.issn 1815-3852
dc.description.abstract A simple and sensitive spectrophotometric method for the quantitative analysis of pyrimethamine (PYM) in pharmaceutical formulations has been described. The method is based on the formation of colored product between PYM and 1,2-naphthoquinone-4-sulfonate (NQS) at 60 °C. The reaction is followed spectrophotometrically by measuring the increase in absorbance at 483 nm as a function of time. Under the optimized reaction condition, Beer’s law correlation for the absorbance (A) with PYM concentration (C) was obeyed in the range 12–40 μg mL−1 the regression equation for the calibration data was A = 0.704 + 0.0132C, with correlation coefficient (0.996). The molar absorptivity (ɛ) was 5.8 × 103 L mol−1 cm−1. The limits of detection and quantification were 3.25 and 10.83 μg mL−1, respectively. The proposed method was successfully applied to the determination of PYM in pharmaceutical tablets with good accuracy and precision; the percentage for PYM was 101.4 ± 1.47% for batch 20 and 100.4 ± 0.51% for batch 13. The results were in good agreement with those obtained with the official high performance liquid chromatography (HPLC) method. en_US
dc.language.iso en en_US
dc.publisher University of Bahrain en_US
dc.rights Attribution-NonCommercial-ShareAlike 4.0 International *
dc.rights.uri *
dc.subject UV–visible spectrophotometry
dc.subject Pyrimethamine
dc.subject NQS
dc.subject Pharmaceutical formulation
dc.title Spectrophotometric determination of pyrimethamine (PYM) in pharmaceutical formulation using 1,2-naphthoquinone-4-sulfonate (NQS) en_US
dc.type Article en_US
dc.source.title Arab Journal of Basic and Applied Sciences
dc.abbreviatedsourcetitle AJBAS

Files in this item

This item appears in the following Issue(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International

All Journals

Advanced Search


Administrator Account